FIGURE 6.

Fully re-differentiated human bronchial epithelial cells (P1 HBECs) from nonsmokers exposed to cigarette smoke (smoke) ± losartan. a) Quantitative mRNA expression of MUC5AC and transforming growth factor (TGF)-β1 in P1 HBECs 24 h after exposure to smoke (24 puffs) without and with losartan (Los) treatment (10 μM throughout differentiation). Data are shown as relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and smoke (n=5 donors for each group). b) Matrix metalloproteinase (MMP)-9 activity assay from PBS washes of P1 HBECs from nonsmokers 24 h after exposure to smoke (24 puffs) without and with Los treatment. Data are shown relative to smoke (n=6 from two donors for each group). c) Left panel: cystic fibrosis transmembrane conductance regulator (CFTR) currents represented by short circuit current changes upon CFTR_inh172 (10 µM) application after 10 µM forskolin stimulation 4 h after exposure to smoke±Los (represented as ΔI_sc upon CFTR_inh172) (n=5 for each group). Right panel: CFTR mRNA expression. Data are shown as relative to GAPDH and smoke (n=6 donors for each group). d) CFTR mRNA expression in air control. Data shown as relative to GAPDH and air (n=3 donors for each group). e) Left panel: voltage-dependent potassium (BK) currents measured upon ATP stimulation 4 h after exposure to smoke±Los (n=5 donors for each group). Right panel: LRRC26 mRNA expression. Data are shown as relative to GAPDH and smoke (n=6 donors for each group). f) LRRC26 mRNA expression in air control. Data are shown as relative to GAPDH and air (n=3 donors for each group). *: p<0.05; all t-tests after passing Shapiro–Wilk normality test.