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. 2021 Jan 25;7(1):00394-2020. doi: 10.1183/23120541.00394-2020

FIGURE 7.

FIGURE 7

Fully re-differentiated human bronchial epithelial cells (P1 HBECs) from active smokers with and without COPD exposed to cigarette smoke (smoke) and treated with losartan (Los). a) Cystic fibrosis transmembrane conductance regulator (CFTR) conductance expressed as a ratio of smoke exposure over air exposure of short circuit current changes upon CFTRinh-172 (10 µM) application after 10 µM forskolin stimulation (ΔIsc smoke/air). Note reverse y-axis similar to figure 3 (more downward=positive=improved conductance) (n=8 from 7 donors). *: p<0.05 using t-test. b) CFTR mRNA expression. Data are shown relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and smoke (n=7 from 7 donors). *: p<0.05 using t-test. c) Voltage-dependent potassium (BK) currents expressed as a ratio of smoke exposure over air exposure of short circuit current changes upon ATP stimulation (ΔIsc smoke/air). Note reverse y-axis (more downward=positive=improved conductance) (n=5 from 5 donors). *: p<0.05 using t-test. d) LRRC26 mRNA expression. Data are shown relative to GAPDH and smoke (n=7 from 7 donors). *: p<0.05 using t-test. e) Ciliary beat frequency (CBF) after exposure to smoke±Los (n=26 from 7 donors). *: p<0.05 using Mann–Whitney U-test. f) Matrix metalloproteinase (MMP)-9 activity assay from apical PBS washes obtained 24 h after exposure to smoke±Los. Data are shown as relative to smoke (≥9 from 7 donors). *: p<0.05 using t-test.