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. 2021 Jan 25;23:39. doi: 10.1186/s13075-021-02423-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — CUR alone, or in combination with a VO-enriched diet, mitigated the abundance of MMPs in the joints of CIA mice. Joint tissue lysates (20 μg protein each) were resolved on NuPage 4–12% Bis-Tris protein gels and probed in immunoblots with antibodies for mouse MMP-1, MMP-3, MMP-9, and MMP-13. Antibody to mouse GAPDH was used to assess protein loading. Densitometry for band intensity was determined using the AmershamTM Imager 680 analysis software version 2.0. Relative band intensity was determined by normalizing to the GAPDH band intensity for each sample. Graphs represent the relative fold change compared to saline-treated mice normalized to 1. GraphPad Prism 7.05 software was used for statistical analyses. Kruskal–Wallis one-way analysis of variance (ANOVA) followed by Dunn’s multiple comparison post hoc test was used to determine the p values (*p ≤ 0.05)