Fig. 1.

Protein level of ULK1 is decreased following MHV-A59 infection.

(A–B) Murine 17Cl1 fibroblasts were infected with MHV-A59 (MOI = 10) for various times as indicated or sham-infected with DMEM. MHV replication was assessed via western blot analysis of NSP9 precursor processing. Cell lysates were harvested and probed for ULK1 using polyclonal anti-ULK1 (A, which recognizes amino acids 351–400) or monoclonal anti-ULK1 (B, which was raised against amino acids 511–750), NSP9, and ACTB. Densitometric analysis of ULK1 protein level was performed with NIH ImageJ, normalized to ACTB and presented underneath as fold changes where the first lane was arbitrarily set to 1. Arrow denotes the cleavage fragment detected at ∼65 kDa. (C) Murine 17Cl1 cells were sham-infected or infected with MHV-A59 (MOI = 10) for 12 h or 24 h. Gene level of Ulk1 was measured by real-time quantitative RT-PCR and normalized to Actb (mean ± SD, n = 3).