Table 2.
Detection Approach | Diagnostic Platforms | Target Analyte Detected | LOD | Analysis Time | References |
---|---|---|---|---|---|
Nucleic acid amplification | qRT-PCR | SARS-CoV-2 E and RdRp genes | 3.9 copies/reaction (E gene); 3.6 copies/reaction (RdRp gene) |
2–4 h | [61] |
RT-LAMP with colorimetric detection | SARS-CoV-2 | 100 copies/reaction | 30–60 min | [62] | |
Detection of viral antibodies and antigens | Capture ELISA immunoassay | anti-SARS-CoV-2 IgG and IgM | 2.384a (IgG); 2.187a (IgM) |
2.5 h | [63] |
ELISA immunoassay | SARS-CoV-2 antigens (N and S proteins) | 100 pg/mL | 3–5 h | [64] |
Fold change over average reading of negative controls.