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. 2020 Dec 31;176:112953. doi: 10.1016/j.bios.2020.112953

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Fig. 5 — Pathogen diagnosis results of clinical blood samples using the BACA assay. Bacteria detection using fluorometric (a) and colorimetric (b and c) BACA biosensor respectively. Results are shown as mean ± s.d. The BoNT/A substrate SNAPtide (FITC/DABCYL) was then added to the assay with a final concentration of 5 μM. Reactions were incubated in a fluorescence plate reader (BioTek H1 microplate reader) with fluorescence read every 5 min as a fluorometric biosensor. The color change of supernatant could be observed to the naked eyes or measured by UV–vis spectrophotometer at 520 nm. All specimens for the tests were measured in triplicate. (NT represents no template control). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)