Figure 2.
Identification of Mitochondrial ADP-Ribosylated Proteins by Mass Spectrometry
(A) Number of peptide spectral matches (PSMs), protein families, and proteins identified to be ADP-ribosylated in mouse muscle tissue (white, total proteins; black, mitochondrial proteins).
(B) Frequency of ADP-ribosylation regions identified on mitochondria-localized proteins.
(C) Schematic overview of the distribution of protein ADP-ribosylation detected in mitochondria from murine muscle. ADP-ribosylation is indicated using a red circle. Each circle represents an ADP-ribsosylated protein or subunit; the size of each circle depicts the number of times the modified peptide/protein was identified by MS, and spectral counts for each protein are indicated in parentheses.
(D) Anti-ADPr antibody stained western blot of whole-cell and mitochondrial lysates isolated from untreated and rotenone-treated U2OS cells stably transfected with HA-tagged OMP25.
(E) WB on mitochondrial lysates from untreated and H2O2-treated cells stained with a commercial pan-ADPr antibody.
(F) WB stained for ADP-ribosylation and ATP5a on mitochondrial lysates isolated from U2OS cells stably transfected with HA-tagged OMP25 (SN, supernatant; PD, pull-down).