REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-ADPr antibody	Hottiger Laboratory	N/A
Anti-pan-ADPr antibody	CST	Cat# E6F6ABF
Anti-PAR antibody	Enzo	Cat# ALX-210-890A-0100
Anti-ADPR eAF1521-FC	Hottiger Laboratory	Nowak et al., 2020
Anti-ATP5a antibody	Abcam	Cat# ab14748
Anti-γH2AX antibody	Biolegend	Cat# 613402
Chemicals, Peptides and Recombinant Proteins
NAD+	Sigma Aldrich	Cat# N7004-1g
ARTD8cat protein, recombinant	Hottiger Laboratory	N/A
ARTD10cat protein, recombinant	Hottiger Laboratory	N/A
ARTD1 protein, recombinant	Hottiger Laboratory	N/A
Dulbecco’s modified Eagle’s medium (DMEM)	ThermoFisher	Cat# 61965-026
Penicillin-streptomycin	ThermoFisher	Cat# 15140-122
Fetal calf serum	ThermoFisher	N/A
Blasticidin	InvivoGen	Cat# ant-bl-1
Hygromycin B	ThermoFisher	Cat# 10687010
Doxycycline hydrate	Sigma	Cat# D9891-1G
OptiMEM	ThermoFisher	Cat# 31985-047
Rotenone	Sigma Aldrich	Cat# R8875-5G
Myxothiazol	Sigma Aldrich	Cat# T5580
Antimycin	Sigma Aldrich	Cat# A8674-25mg
Oligomycin	Calbiochem	Cat# 495455
FCCP	Sigma Aldrich	Cat# C2910-10MG
PJ34	Adoog	Cat# A12665-50
Olaparib	Selleckchem	Cat# S1060
Talazoparib	Selleckchem	Cat# S7048
Niraparib	Adooq	Cat# A11026
Methyl Methanesulfonate	Sigma Aldrich	Cat# 129925-25G
Hydrogen peroxide solution	Sigma Aldrich	Cat# H1009-100ml
DAPI	BioLegend	Cat# 422801
Bovine serum albumin	Sigma Aldrich	Cat# A9418
Formaldehyde solution	Sigma Aldrich	Cat# F8775
Mowiol 4.88	Calbiochem	Cat# 475904
SiR-Halo	Lukinavičius et al., 2013	N/A
CP-TMR-C6-SMX	Sallin et al., 2018	N/A
Critical Commercial Assays
Lipofectamine RNAiMAX	ThermoFisher	Cat# 13778-150
MultiScribe Reverse Transcriptase	ThermoFisher	Cat# 4311235
KAPA Biosystems Sybr Fast qPCR Kit	Roche	Cat# KK4600
Flp-In™ TRex™ Core Kit	ThermoFisher	Cat# K650001
Experimental Models: Cell Lines
U-2 OS, human (female origin)	ATCC	Cat# HTB-96
C2C12, myoblasts, mouse	ATCC	Cat# CRL-1772
HeLa cells (female origin)	ATCC	Cat# CCL-2
U2OS 3xHA-EGFP-OMP25	This paper	N/A
U2OS Flp-In nuclear NAD+ sensor; inducible	This paper	N/A
U2OS Flp-In cytoplasmic NAD+ sensor; inducible	This paper	N/A
U2OS Flp-In mitochondrial NAD+ sensor; inducible	This paper	N/A
Experimental Models: Organisms/strains
C57BL/6N	Wellcome Trust Sanger Institute	N/A
Recombinant DNA
pMXs-3xHA-EGFP-OMP25	Addgene	Cat# 83356
pCMV-VSV-G	Addgene	Cat# 8454
pUMVC	Addgene	Cat# 8449