KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal to PPAR gamma | Abcam | Cat# ab209350 |
| Rabbit polyclonal to Adipose Triglyceride Lipase (phosphor S406) | Abcam | Cat# ab135093 |
| Total OXPHOS Rodent WB Antibody Cocktail | Abcam | Cat# ab110413; RRID: AB_2629281 |
| Rabbit polyclonal to PGC1 alpha + beta | Abcam | Cat# ab72230; RRID: AB_1640773 |
| ApoE (pan) (D719N) Rabbit mAb | Cell Signaling Technology | Cat# 13366; RRID: AB_2798191 |
| Akt (pan) (C67E7) Rabbit mAb | Cell Signaling Technology | Cat# 4691S; RRID: AB_915783 |
| Phospho-Akt (Ser473) (D9E) XP Rabbit mAb | Cell Signaling Technology | Cat# 4060S; RRID: AB_2315049 |
| OPA1 (D6U6N) Rabbit mAb | Cell Signaling Technology | Cat# 80471; RRID: AB_2734117 |
| DRP1 (D6C7) Rabbit mAb | Cell Signaling Technology | Cat# 8570; RRID: AB_10950498 |
| SREBP-1 (2A4): sc-13551 | Santa Cruz Biotechnology | Cat# sc-13551; RRID: AB_628282 |
| SNAP25 (D7B4) Rabbit mAb | Cell Signaling Technology | Cat# 5308; RRID: AB_10621948 |
| PSD95 (D74D3) Rabbit mAb | Cell Signaling Technology | Cat# 3409; RRID: AB_1264242 |
| Hexokinase I (C35C4) Rabbit mAb | Cell Signaling Technology | Cat# 2024; RRID: AB_2116996 |
| Hexokinase II (C64G5) Rabbit mAb | Cell Signaling Technology | Cat# 2867; RRID: AB_2232946 |
| Glial fibrillary acidic protein antibody | MED Millipore | Cat# MAB360; RRID: AB_11212597 |
| TOMM20 antibody | Abcam | Cat# ab186734; RRID: AB_2716623 |
| Monoclonal Anti-MAP2 antibody | Sigma | Cat# M4403; RRID: AB_477193 |
| Perilipin-2/ADFP antibody | Novus Biologicals | Cat# NB110-40877; RRID: AB_787904 |
| ApoE4 antibody | Novus Biologicals | Cat# NBP1-49529; RRID: AB_10011579 |
| Phospho-AMPKα (Thr172)(40H9) Rabbit mAb | Cell Signaling Technology | Cat# 2535; RRID: AB_331250 |
| AMPKα (D63G4) Rabbit mAb | Cell Signaling Technology | Cat# 5832; RRID: AB_10624867 |
| Acetyl-CoA Carboxylase Antibody | Cell Signaling Technology | Cat# 3662; RRID: AB_2219400 |
| Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody | Cell Signaling Technology | Cat# 3661; RRID: AB_330337 |
| β-actin (8H10D10) Mouse mAb | Cell Signaling Technology | Cat# 3700; RRID: AB_2242334 |
| Fatty Acid Synthase (C20G5) Rabbit mAb | Cell Signaling Technology | Cat# 3180; RRID: AB_2100796 |
| Mouse Anti-PDH-E1α Antibody | Mitoscience | Cat# MSP07; RRID: AB_478296 |
| PhosphoDetect Anti-PDH-E1α (pSer293) Rabbit pAb | Sigma | Cat# AP1062; RRID: AB_10616069 |
| Goat anti-rabbit IgG (H+L) | Vector Laboratories | Cat# PI-1000; RRID: AB_2336198 |
| Goat anti-mouse IgG (H+L) | Vector Laboratories | Cat# PI-2000; RRID: AB_2336177 |
| Alexa Fluor 488 goat anti-mouse | Thermo Fisher Scientific | Cat# A11001; RRID: AB_2534069 |
| Alexa Fluor 488 goat anti-rabbit | Thermo Fisher Scientific | Cat# A11008; RRID: AB_143165 |
| Alexa Fluor 555 goat anti-mouse | Thermo Fisher Scientific | Cat# A21422; RRID: AB_2535844 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| BODIPY FL C16 | Thermo Fisher Scientific | Cat# D3821 |
| BODIPY 558/568 C12 | Thermo Fisher Scientific | Cat# D3835 |
| BODIPY 581/591 C11 | Thermo Fisher Scientific | Cat# D3861 |
| HCS LipidTOX™ Green Neutral Lipid Stain | Thermo Fisher Scientific | Cat# H34475 |
| HCS LipidTOX™ Red Neutral Lipid Stain | Thermo Fisher Scientific | Cat# H34476 |
| DMEM/F-12, HEPES, no phenol red | Thermo Fisher Scientific | Cat# 11039047 |
| HBSS, no calcium, no magnesium | Thermo Fisher Scientific | Cat# 14170112 |
| DPBS | Thermo Fisher Scientific | Cat# 14287080 |
| B-27 Plus Neuronal Culture System | Thermo Fisher Scientific | Cat# A3653401 |
| Fetal Bovine Serum | ATCC | Cat# 302020 |
| 3-methyladenine (3-MA) | Sigma | Cat# M9281-100MG |
| XF Palmitate-BSA FAO Substrate | Agilent | Cat# 102720-100 |
| MitoTracker Green FM | Thermo Fisher Scientific | Cat# M7514 |
| ND-630 | Bio Vision | Cat# B2248-5 |
| Triacsin C | Cayman Chemical | Cat# 10007448500 |
| Atglistatin | Cayman Chemical | Cat# 1469924-27-3 |
| Pitstop 2 | Sigma | Cat# SML1169 |
| Adenosine 5’-diphosphate sodium salt | Sigma | Cat# A2754-1G |
| TaqManFast Advanced Master Mix | Thermo Fisher Scientific | Cat# 4444557 |
| RTA Midi PVDF Transfer Kit | Bio-Rad | Cat# 1704273 |
| 4–15% Criterion TGX Precast MidProtein Gel | Bio-Rad | Cat# 5671085 |
| Oligomycin A | Sigma | Cat# 75351-5MG |
| Methyl arachidonyl fluorophosphonate (MAFP) | Cayman Chemical | Cat# 706601010MG |
| Recombinant Human ApoE3 | Fitzgerald Industries | Cat# 30R-2381 |
| Recombinant Human ApoE4 | Fitzgerald Industries | Cat# 30R-2382 |
| Critical Commercial Assays | ||
| Seahorse XF Mito Fuel Flex Test Kit | Agilent | Cat# 103260-100 |
| Lactate Colorimetric/Fluorometric Assay Kit | Bio Vision | Cat# K607-100 |
| Triglyceride Quantification Colorimetric/Fluorometric Kit | Bio Vision | Cat# K622-100 |
| Free Fatty Acid Fluorometric Assay Kit | Cayman Chemical | Cat# 700310-96 |
| Glucose Uptake Cell-Based Assay Kit | Cayman Chemical | Cat# 600470 |
| Adult Brain Dissociation Kit | Miltenyi Biotec | Cat# 130-107-677 |
| ATP Determination Kit | Thermo Fisher Scientific | Cat# A22066 |
| Low Density Lipoprotein Uptake Kit | Thermo Fisher Scientific | Cat# I34359 |
| Lipid Extraction Kit (Chloroform Free) | Abcam | Cat# ab211044 |
| Lipid Assay Kit (unsaturated fatty acids) | Abcam | Cat# ab242305 |
| Experimental Models: Organisms/Strains | ||
| Mouse: B6(SJL) -Apoeem2(ApoE*)Adiuj/J | The Jackson Laboratory | Stock No: 029018 |
| Mouse: B6(SJL)-Apoetm1.1(ApoE*4)Adiuj/J | The Jackson Laboratory | Stock No: 027894 |
| Mouse: C57BL/6J | The Jackson Laboratory | Stock No: 000664 |
| Oligonucleotides | ||
| APOE (Hs00171168_m1) | Life Technologies | Cat# 4331182 |
| Actb (Mm00607939_s1) | Life Technologies | Cat# 4331182 |
| Software and Algorithms | ||
| GraphPad Prism 9 | GraphPad Software | https://www.graphpad.com/ |
| Imaris 9.3.0 | Oxford Instruments | https://imaris.oxinst.com/ |
| Image Lab 6.0.1 | Bio-Rad | https://www.bio-rad.com/ |
| MetaboAnalyst 4.0 | Chong et al., 2019 | https://www.metaboanalyst.ca |
| BioRender | BioRender | https://biorender.com |
| Micro-P Software | Peng et al., 2011 | N/A |