KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Stains
BL21 Gold DE3 competent cells	Stratagene	Cat # 230132
Chemicals, Peptides, and Recombinant Proteins
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)	Avanti Polar Lipids	Cat # 850457C
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE),	Avanti Polar Lipids	Cat # 850757C
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine (POPS)	Avanti Polar Lipids	Cat # 840034C
Cholesterol	Avanti Polar Lipids	Cat # 700000P
N-(7-nitro-2,1,3-benzoxadiazole-4-yl)-1,2-dipalmitoyl phosphatidylethanolamine (NBD-DPPE)	Avanti Polar Lipids	Cat # 810114C
N-(Lissamine rhodamine B sulfonyl)-1,2-dipalmitoyl phosphatidylethanolamine (rhodamine-DPPE)	Avanti Polar Lipids	Cat # 810158C
Nycodenz	Axis-Shield	Cat # 1002424
Protease inhibitor cocktail	Roche	Cat # 05056489001
CHAPSO (3-((3-Cholamidopropyl) dimethylammonio)-2-hydroxy-1-propanesulfonate)	Soltec Ventures	Cat # 82473-24-3
CHAPS (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate)	Sigma-Aldrich	Cat # C3023
OG (n-Octyl-β-D-glucopyranoside)	EMD Millipore	Cat # 494459
Ficoll 70	GE	Cat # 17-0310-10
Recombinant DNA
pET28a	Novagen	Cat # 69864-3
pET15b	Novagen	Cat # 69661-3
pTW34	(Weber et al., 2000)	N/A
pET-SUMO-Munc18–1	Shen et al., 2007	Cat # 135550 in Addgene
pET-SUMO-VAMP2	Shen et al., 2007	Cat # 135551 in Addgene
pET-syntaxin-4	Yu et al., 2013	N/A
pET-SNAP-23	Yu et al., 2013	N/A
pET-SUMO-VAMP8	Yu et al., 2019	Cat # 135553 in Addgene
Software and Algorithms
KaleidaGraph	Synergy	https://www.synergy.com/wordpress_650164087/kaleidagraph/