(A) Kaplan-Meier survival curve of mice implanted with 4121 or 3832 GSCs transduced with shNT or shPiwil1 shRNAs (n = 12 mice for each group).
(B) Immunofluorescence staining of Piwil1 and cleaved caspase 3 in shNT and shPiwil1 mice. Representative data from 3832 GSC-derived xenografts are shown. Nuclei were counterstained with DAPI (blue).
(C) Immunofluorescence staining of BTG2, FBXW7, and MCL1 in shNT and shPiwil1 mice. Representative data from 3832 GSC-derived xenografts are shown. Nuclei were counterstained with DAPI (blue).
****p < 0.0001.