KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-BTG2 | Santa Cruz | Cat#sc-517187 |
| Rabbit monoclonal anti-CCND2 | Novus Biologicals | Cat# NBP2-14460 |
| Mouse monoclonal anti-CD31 | Abcam | Cat#ab187377, RRID:AB_2756834; lot#GR317844-4 |
| Rabbit monoclonal anti-Cleaved-Caspase 3 | Cell Signaling Technology | Cat#9664, RRID: AB_10891784‘ |
| Rabbit monoclonal anti-Cleaved-PARP | Cell Signaling Technology | Cat#5625S, RRID: AB_10699459; lot#13 |
| Rabbit polyclonal anti-FBXW7 | Abcam | Cat#ab109617; RRID:AB_2687519; lot#GR190912-1 |
| Mouse monoclonal anti-Flag | Sigma-Aldrich | Cat#F1804, RRID: AB_262044 |
| Mouse monoclonal anti-GAPDH | Santa Cruz | Cat# sc-47724, RRID:AB_627678; lot#F2216 |
| Rabbit polyclonal anti-GFAP | Agilent | Cat#Z0334, RRID:AB_10013382; lot#00085136 |
| Mouse monoclonal Human Nuclei antibody | Millipore | Cat#MAB1281, RRID:AB_94090; lot#3129247 |
| Rabbit polyclonal anti-MCL1 | Santa Cruz | Cat# sc-819; RRID:AB_2144105 |
| Mouse monoclonal anti-Nestin | BD Biosciences | Cat# 611658, RRID:AB_399176; lot#13748 |
| Mouse monoclonal anti-Olig2 | Millipore | Cat# MABN50, RRID:AB_10807410; lot#3109744 |
| Rabbit polyclonal anti-p27 | Cell Signaling Technology | Cat# 2552, RRID:AB_10693314; lot#7 |
| Rabbit polyclonal anti-Piwil1 | Abcam | Cat#ab12337; RRID:AB_470241; lot#GR312421-1 |
| Mouse monoclonal anti-Sox2 | Millipore | Cat#MAB4423, RRID: AB_11213224; lot#3112625 |
| Mouse monoclonal anti-α-Tubulin | Sigma-Aldrich | Cat# T6199, RRID:AB_477583 |
| Biological Samples | ||
| GBM specimens | Cleveland Clinic Brain Tumor Bank | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| EGF | R&D | Cat#236-EG |
| FGF | R&D | Cat#4114-TC |
| Actinomycin D | Invitrogen | Cat#A7592 |
| Critical Commercial Assays | ||
| Cell Titer-Glo | Promega | Cat#G7572 |
| Mouse and Rabbit Specific HRP/AEC IHC Detection Kit | Abcam | Cat#236467 |
| Senescence β-Galactosidase Staining Kit | Cell Signaling Technology | Cat#9860S |
| Click-iT EdU Imaging Kits | Thermo Fisher | Cat#C10338 |
| Transwell polycarbonate membrane cell culture inserts | Corning | Cat#3422 |
| SuperScript IV VILO Master Mix | Thermo Fisher | Cat# 11756050 |
| PowerUp SYBR Green Master Mix | Thermo Fisher | Cat#A25742 |
| Deposited Data | ||
| Raw and analyzed data | This paper | GEO: GSE160437 |
| Experimental Models: Cell Lines | ||
| 293T | ATCC | Cat# CRL-3216, RRID:CVCL_0063 |
| 4121 | Gifts from Dr. Jeremy Rich | N/A |
| 3832 | Gifts from Dr. Jeremy Rich | N/A |
| 387 | Gifts from Dr. Jeremy Rich | N/A |
| 3359 | Gifts from Dr. Jeremy Rich | N/A |
| Experimental Models: Organisms/Strains | ||
| NSG mice | The Jackson Laboratory | Cat# 005557 |
| Oligonucleotides | ||
| qPCR primers, see Table S1 | This paper | N/A |
| Recombinant DNA | ||
| pCDH-MCS-T2A-Puro-MSCV lentiviral vector | System Biosciences | Cat#CD522A-1 |
| pCDH-EF1 α-MCS-IRES-neo lentiviral vector | System Biosciences | Cat#CD532A-2 |
| pMSCV-Flag-Piwil1 | This paper | N/A |
| pEF1 α-Flag-MCL1 | This paper | N/A |
| pEF1 α-Flag-OLIG2 | This paper | N/A |
| pEF1 α-Flag-CCND2 | This paper | N/A |
| shRNA human pLKO.1 CTRL shRNA | Sigma-Aldrich | Cat# SHC002 |
| shRNA human pLKO.1 Piwil1 shRNA#1 | Sigma-Aldrich | TRCN0000417558 |
| shRNA human pLKO.1 Piwil1 shRNA#2 | Sigma-Aldrich | TRCN0000007878 |
| Software and Algorithms | ||
| GraphPad Prism 8 | GraphPad Software | RRID:SCR_002798; https://www.graphpad.com/ |
| ImageJ | Schneider et al., 2012 | RRID:SCR_003070; https://imagej.nih.gov/ij/ |
| ELDA | Hu and Smyth, 2009 | RRID:SCR_018933; http://bioinf.wehi.edu.au/software/elda/ |
| Modfit LT | Verify Software House | RRID:SCR_016106; http://www.vsh.com/products/mflt/ |
| R Bioconductor package: Beadarray 2.36.1 | Dunning et al., 2007 | RRID:SCR_001314; https://bioconductor.org/packages/release/bioc/html/beadarray.html |
| R Bioconductor package: clusterProfiler 3.14.3 | Yu et al., 2012 | RRID:SCR_016884; https://bioconductor.org/packages/release/bioc/html/clusterProfiler.html |
| R Bioconductor package: limma 3.42.2 | Ritchie et al., 2015 | RRID:SCR_010943; https://bioconductor.org/packages/release/bioc/html/limma.html |