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. 2021 Jan 22;2(1):e25995. doi: 10.2196/25995

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©Emilio Mastriani, Alexey V Rakov, Shu-Lin Liu. Originally published in JMIR Research Protocols (http://www.researchprotocols.org), 22.01.2021.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work, first published in JMIR Research Protocols, is properly cited. The complete bibliographic information, a link to the original publication on http://bioinform.jmir.org, as well as this copyright and license information must be included.

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Topology diagram using the MemBrain v3. The illustration depicts the transmembrane topology of the sequence and highlights that the amino acid at position 50 (G) is positioned into the middle of the transmembrane region. Red: transmembrane helix (TMH); blue: loop.