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. 2021 Jan 22;2(1):e25995. doi: 10.2196/25995

Table 2.

Protein disorder analysis results showing the probability of disorder for each position of the protein. The probabilities have been calculated using MFDp2, Netsurf, and SPOTD software.

Position Amino acid sequence Disorder probability values


MFDp2 NetsurfP2 SPOTD Average valuea
1 M 0.132 0.627823114 0.5607 0.440174371
2 L 0.134 0.347978383 0.5358 0.339259461
3 Q 0.135 0.270706475 0.4945 0.300068825





39 T 0.03 0.010842944 0.1936 0.078147648
40 V 0.029 0.007660664 0.189 0.075220221
41 Q 0.027 0.004478907 0.172 0.067826302
42 E 0.025 0.00340931 0.1848 0.07106977
43 I 0.025 0.003887762 0.1968 0.075229254
44 Q 0.024 0.003997837 0.1927 0.073565946
45 L 0.023 0.00361518 0.2129 0.079838393
46 Q 0.023 0.004551574 0.2123 0.079950525
47 A 0.023 0.004939525 0.2011 0.076346508
48 A 0.022 0.005752307 0.2133 0.080350769
49 V 0.022 0.002826149 0.2524 0.092408716
50b G 0.022 0.005828088 0.2013 0.076376029
51 E 0.022 0.001046103 0.24 0.087682034
52 L 0.023 0.000922468 0.2694 0.097774156
53 L 0.023 0.001263275 0.2588 0.094354425
54 L 0.023 0.001187441 0.2539 0.092695814
55 L 0.023 0.000650476 0.2483 0.090650159
56 E 0.023 0.000615434 0.2328 0.085471811
57 W 0.023 0.001080571 0.2302 0.08476019
58 L 0.023 0.000941573 0.2154 0.079780524
59 A 0.023 0.001573079 0.208 0.07752436
60 M 0.024 0.000997698 0.2853 0.103432566
61 A 0.024 0.00227783 0.3026 0.109625943
62 V 0.025 0.003362786 0.3503 0.126220929

aAverage values of the disorder probability for each position.

bAmino acid G placed at position 50, inside the stable region.