Table 3.

Transmembrane prediction results obtained using TMHMM, MemBrainTHM, ProtScale, and TMpred applications. Results from ProtScale and TMpred have been normalized for comparison with other probabilities.

Position	Amino acid sequence	TMHMM probability	MemBrain THM propensity	ProtScale normalized score	TMpred normalized score	Transmembrane probability, average valuea
1	M	0	0.000191	N/Ab	0.661425764	0.220538921
2	L	0	0.002851	N/Ab	0.661425764	0.221425588
3	Q	0	0.046538	N/Ab	0.661425764	0.235987921
…
49	V	0.2594	0.987914	0.646	0.603358942	0.624168236
50c	G	0.27719	0.987914	0.646	0.629801679	0.63522642
51	E	0.28083	0.991702	0.736	0.660532428	0.667266107
52	L	0.32735	0.993857	0.67	0.594246918	0.646363479
53	L	0.56651	0.993857	0.637	0.778452743	0.743954936
54	L	0.63937	0.994522	0.632	0.73360729	0.749874822
55	L	0.64032	0.990459	0.659	0.818831517	0.777152629
56	E	0.64052	0.96027	0.726	0.835626228	0.790604057
57	W	0.64826	0.946819	0.701	0.822583527	0.779665632
58	L	0.6493	0.947424	0.706	0.895122387	0.799461597
59	A	0.64928	0.947424	0.683	0.905663748	0.796341937
60	M	0.64927	0.970735	0.683	0.947293193	0.812574548
61	A	0.64924	0.970735	0.773	0.955511881	0.83712172
62	V	0.64903	0.937507	0.831	1	0.85438425
63	M	0.64893	0.892506	0.831	0.960871896	0.833326974
64	L	0.6482	0.846403	0.84	0.942826514	0.819357379
65	L	0.64758	0.781733	0.847	0.924066464	0.800094866
66	L	0.63557	0.670387	0.856	0.661425764	0.705845691
67	L	0.61835	0.539353	0.851	0.661425764	0.667532191
68	C	0.5428	0.455615	0.819	0.661425764	0.619710191
69	C	0.51009	0.430385	0.728	0.661425764	0.582475191
70	C	0.44702	0.380525	N/Ab	0.661425764	0.496323588

^aAverage values of the probability for each position.

^bThe window size used for the profile computation is 9, so the score is not applicable for positions 1-4 and 70-73.

^cAmino acid G placed at position 50, inside the stable region.