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Intact protein mass spectrometry for exchange of covalently bound ETX1317 (273 Da) from KPC-2 (28 720 Da) to OXA-48 (28 280 Da) after 19 h. Shown are the mass spectra of untreated KPC-2 (black), the KPC-2–ETX1317 covalent complex (blue; 28 993 Da), the mixture of untreated KPC-2 and OXA-48 (green), and the mixture of KPC-2–ETX1317 covalent complex with OXA-48 (red). The mass of the OXA-48-ETX1317 covalent complex is 28 553 Da.
