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. 2021 Jan 4;10:e63907. doi: 10.7554/eLife.63907

Figure 7. Classification and characterization of RGCs.

(A) Clustering of E16 RGCs using UMAP. (B) Dot plots showing expression of selected genes expressed in all or subsets of RGCs. Numbers correspond to clusters in A. Dendrogram above dots shows transcriptional relationships of clusters. (C) Dot plots showing expression of genes selectively expressed in RGC clusters. (D, E) Immunostaining of E16 retina for SATB2 and BRN3A/POU4F1 (D) and for SATB1 and SATB2 (E). (F–H) RGCs labeled by eCHIKIN-mediated GFP insertion in TFAP2D (GC23; F), MC5R (GC18; G), and ETV1 (GC15; H). Bars in E and H, 10 µm.

Figure 7.

Figure 7—figure supplement 1. Frequency distribution and morphological analysis of retinal ganglion cell types.

Figure 7—figure supplement 1.

(A) Frequency distribution of RGCs. Cluster numbers are from Figure 7A,B. (B–I) In situ hybridization of E16 retina for MODX1 in GC34 (B), LOC419389 in GC32 (C), ZIC1 in GC3 (D), ETV1 in GC15 or 21 (E), RUNX1 in GC36 (F), RUNX2 in GC1 (G), MC5R in GC23 (H), and OPN4.1 in GC41 (I). Bar, 10 µm.
Figure 7—figure supplement 2. Markers expressed and co-expressed by key retinal ganglion cell types.

Figure 7—figure supplement 2.

Double-color in situ hybridization on E16 retina for RGC markers. Blue, DAPI.