Figure 4.

ROS was involved in necroptosis induced by emodin in renal cancer cells. (a) Emodin-induced cell viability inhibition of 786-O and OS-RC-2 cells could be reversed after pretreatment by 10 μM NAC for 6 h. (b) NAC treatment could reduce emodin-induced necrosis. Representative density plots of flow cytometric analysis on the fraction of dead cells 24 h after the indicated treatments detected with Annexin V/propidium iodide. (c) The histogram represents the mean values of three independent experiments. (d) Western blot analysis of P-RIP1 and P-MLKL in 786-O and OS-RC-2 cells under the described condition.