Figure 7.

Emodin induced necroptosis through increased ROS in a JNK-dependent pathway. (a) The level of p-JNK was examined by western blot in 786-O and OS-RC-2 cells treatment with different concentrations of emodin (0, 10, 25, 50, and 100 μM, respectively) for 24 h. (b) Western blot analysis of p-JNK level in 786-O and OS-RC-2 cells. The cells were treated with 0, 25, and 50 μM emodin for 24 h, respectively, with or without NAC pretreatment for 6 h. (c) Effect of JNK inhibitor SP600125 on p-JNK, p-RIP1, and p-MLKL by western blot analysis. 786-O and OS-RC-2 cells were treated with 50 μM emodin for 24 h with SP600125 pretreatment (0, 5, 10, 20, and 40 μM, respectively) for 3 h.