Figure 8.

Emodin inhibited glycolysis by downregulation of GLUT1 through ROS-mediated PI3K/AKT signaling pathway. (a, c, d) 786-O and OS-RC-2 cells were treated with different concentrations of emodin (0, 10, 25, 50, and 100 μM, respectively) for 24 h. The expression levels of GLUT1, p-P85, P85, P110α, P110β, P110γ, p-AKT, and AKT were tested by western blot. (b, e) Western blot analysis of GLUT1 and p-AKT levels in 786-O and OS-RC-2 cells. The cells were treated with 0, 25, and 50 μM emodin for 24 h, respectively, with or without NAC pretreatment for 6 h. (f) Effect of AKT activator SC79 on p-AKT and GLUT1 by western blot analysis. 786-O and OS-RC-2 cells were treated with 50 μM emodin for 24 h with SC79 pretreatment (0, 5, 10, 20, and 40 μM, respectively) for 3 h.