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. 2021 Jan 25;58(1):64–69. doi: 10.2141/jpsa.0190135

Fig. 3.

Fig. 3.

EYA3 mRNA containing exon 7 in the quail pars tuberalis under the SD and 1LD conditions. PCR was performed to amplify regions from exons 6 to 10 of EYA3 mRNA. The primer position is shown in Fig. 2 and the probe for Southern blotting was 92 bp of EYA3 exon 7. Each bar represents the mean±SEM (n=5). The upper panel of the graph shows the signal containing exon 7 of EYA3 detected by chemiluminescence. SD and 1LD indicate short daycondition and first long daycondition, respectively. a: Expression of exons 6, 7, and 10 (360 bp) under the SD and 1LD conditions. b: Expression of exons 6, 7, 8, 9, and 10 (627 bp) under the SD and 1LD conditions. Asterisks indicate significant difference (P<0.01).