Table 8. Effect of dietary B. licheniformis-fermented product supplementation on mRNA expression in spleens of broilers challenged with C. perfringens.
C1 | E2 | L3 | H4 | SEM | P value | |
---|---|---|---|---|---|---|
22 d | ||||||
inos5 | 1.06 | 0.3 | 0.8 | 1.0 | 0.10 | 0.057 |
cox2 | 1.0ab | 0.2a | 1.7b | 1.6b | 0.21 | 0.002 |
ifnγ | 1.0c | 0.1d | 1.6b | 2.1a | 0.22 | <0.001 |
il-1β | 1.0b | 2.1ab | 2.9ab | 3.6a | 0.35 | 0.017 |
35 d | ||||||
inos | 1.0b | 0.5b | 2.2a | 1.1b | 0.19 | 0.001 |
cox2 | 1.0ab | 0.4b | 1.1ab | 1.4a | 0.14 | 0.036 |
ifnγ | 1.0ab | 0.8b | 1.3ab | 1.4a | 0.09 | 0.002 |
il-1β | 1.0b | 1.5ab | 2.5a | 2.5a | 0.23 | 0.022 |
C=Basal diet plus C. perfringens challenge.
E=Basal diet plus C. perfringens challenge and 0.5 g/kg enramycin from 1 to 21 d and 1.2 g/kg enramycin from 22 to 35 d, respectively.
L=Basal diet plus C. perfringens challenge and 1 g/kg B. licheniformis-fermented products.
H=Basal diet plus C. perfringens challenge and 2 g/kg B. licheniformis-fermented products.
inos=inducible nitric oxide synthase; cox2=cyclooxygenase 2; ifnγ=interferon γ; il-1β=interleukin 1β.
Three replicates were performed per group (n=3).
Different letters indicate a statistically significant difference between groups (P<0.05).