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. 2021 Jan 22;100(3):e23423. doi: 10.1097/MD.0000000000023423

Figure 4.

Figure 4

LncRNA NEAT1 promotes proliferation of OC cells and angiogenesis of HUEVCs by regulating FGF9. Note: After SKOV3 cells and OVCAR-3 cells were transfected with sh-NEAT1, pcDNA3.1-NEAT1, sh-FGF9, or pcDNA3.1-NEAT1+sh-FGF9, (A) qRT-PCR and (B) Western blot tested the expression of FGF9. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, compared to sh-NC group; #P < 0.05, compared to pcDNA3.1 group; & P < 0.05, && P < .01, compared to sh-FGF9 group. After SKOV3 cells and OVCAR-3 cells were transfected with sh-FGF9 or pcDNA3.1-NEAT1+sh-FGF9, (C) CCK8 detected cell proliferation activity; (D) clone formation assay measured the number of cell colonies; (E) Matrigel angiogenesis assay tested the number of vascular branches formed by HUEVCs; (F) qRT-PCR and (G) Western blot analyzed the expressions of VEGF, Ang-1 and MMP2 in HUEVCs. ∗P < .05, ∗∗P < .01, compared to sh-NC group; #P < .05, ##P < 0.01, compared to sh-FGF9 group; qRT-PCR, quantitative reverse transcript polymerase chain reaction; HUEVCs, human umbilical vein endothelial cells.