Dynamic change of SIRT6 expression is related to APAP injury-responsive liver repair. (A) Male C57BL/6 mice were administered a single dose of 400 mg/kg APAP by intraperitoneal injection and killed at 0, 6, 12, 24, 48 and 72 h after APAP treatment (n = 6). (B) H&E-stained liver sections, (C) serum ALT and (D) AST activities from APAP-treated mice over a time course of 0–72 h after 400 mg/kg APAP challenge (n = 6). (E) Western blot analysis of total and nuclear SIRT6 protein expression in APAP-treated mice at various time points after APAP challenge. (F) Densitometric analysis of Western blots (n = 3−4). Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 versus 0 h.