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. 2020 Jul 4;11(1):89–99. doi: 10.1016/j.apsb.2020.06.016

Figure 3.

Figure 3

Knockdown of Sirt6 inhibited hepatocellular proliferation in AML12 cells exposed to APAP. (A) Cell viability assay was determined using Cell Counting Kit-8 in AML12 cells after Sirt6 siRNA transfection and APAP treatment. (B) Proliferation capacity of AML12 cells was investigated by BrdU assay. (C) Colony formation assay of AML12 cells was stained by Diff-Quick after cultured for 7 days. (D) Western blot and (E) densitometric analysis of CCNA1, CCND1 and CDK4 proteins were measured. Data are expressed as the mean ± SD, n = 5. *P < 0.05, **P < 0.01, ***P < 0.001 versus the untreated group; #P < 0.05, ##P < 0.01, ###P < 0.001 versus the control siRNA group.