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. 2020 Jul 4;11(1):89–99. doi: 10.1016/j.apsb.2020.06.016

Figure 4.

Figure 4

Effects of knockdown of Sirt6 on the expressions of NRF2 and its downstream genes. Western blot (A) and densitometric analysis (B) of total NRF2, nuclear NRF2, and pNRF2 in AML12 cells were performed after transfected with Sirt6 siRNA for 24 h and further treated with APAP for 24 h. (C) qRT-PCR analysis was performed to measure the Nrf2, Ho-1, Gstα, Gstμ mRNA level after transfection with Sirt6 siRNA and further treated with APAP for 12 and 24 h, respectively. (D) Dynamic changes of Ho-1 mRNA expression were measured after transfection with Sirt6 siRNA and further treated with APAP. Data are expressed as the mean ± SD, n = 5. *P < 0.05, **P < 0.01, ***P < 0.001 versus the untreated group; #P < 0.05, ##P < 0.01, ###P < 0.001 versus the control siRNA group.