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. 2020 Jul 13;11(1):127–142. doi: 10.1016/j.apsb.2020.07.002

Figure 4.

Figure 4

Anti-VEGFB/IL22 fusion protein inhibited NLRP3 inflammasome activation, alleviated ROS generation and reduced renal fibrosis in db/db mice. (A) The levels of ROS and mitochondrial membrane potential in the kidney sections were measured by Mitosox staining and JC-1 assay kit (scale bar: 100 μm). (B)–(C) The levels of NIRP3, cleaved caspase-1 and mature IL-1β and the levels of vimentin, α-SMA and collagen IV were measured by Western blotting. (D) The statistical assessment of the levels of ROS and JC-1 aggregates/monomers (n = 3–5). (E) and (H) Representative Masson images of mouse kidney samples and the assessment of Masson-positive staining (scale bar: 100 μm) (n = 3–5). (F) Immunohistochemical images of TNF-α and NF-κB P65 in kidney sections (scale bar: 50 μm). (G) and (I) Quantitative analysis of expression of NLRP3, cleaved caspase-1 and mature IL1-β, and the expression of vimentin, α-SMA and collagen IV (n = 3–5). ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001.