Skip to main content
. 2021 Jan 13;11:573524. doi: 10.3389/fphar.2020.573524

FIGURE 2.

FIGURE 2

Inhibition of MYH9 repressed TGF-β1-induced fibroblast-to-myofibroblast differentiation in MRC-5 cells (A) RNA interference-mediated knockdown of MYH9 repressed TGF-β1-induced fibroblast-to-myofibroblast differentiation in MRC-5 cells. MRC-5 cells were transfected with scramble or MYH9 siRNA (50 nM); 24 h after transfection, the cells were starved overnight. MRC-5 cells were treated with/without 2.5 ng/ml of TGF-β1 for 48 h. MRC-5 cell protein samples were prepared and assayed by Western blot analysis for determing MYH9, α-SMA and fibronectin protein expression levels (B) Blebbistatin alleviated TGF-β1-stimulated differentiation of MRC-5 cells. MRC-5 cells were co-treated with/without 2.5 ng/ml of TGF-β1 and 10 μM of blebbistatin for 48 h. The α-SMA protein expression levels in different treatment groups were determined by Western blot analysis. n = 3; scramble siRNA group vs. scramble siRNA group treated with TGF-β1: *p < 0.05; scramble siRNA group treated with TGF-β1 vs. MYH9 siRNA group treated with TGF-β1: # p < 0.05.