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Smad2/3 mediated TGF-β1-stimulated MYH9 expression in MRC-5 cells. MRC-5 cells were transfected with scramble, Smad2, or Smad3 siRNA (100 nM); 24 h later, the cells were starved overnight. The cells were treated with/without 2.5 ng/ml of TGF-β1 for 48 h. MRC-5 cell protein samples were prepared and assayed by Western blot analysis for determing MYH9 protein expression levels (n = 3; control group vs. TGF-β1 group: *p < 0.05; scramble siRNA group vs. Smad siRNA group: # p < 0.05).
