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. 2021 Jan 13;11:573524. doi: 10.3389/fphar.2020.573524

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Copyright © 2021 Sun, Zhu, Chen and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockdown of MYH9 did not change the Smad2/3 phosphorylation in TGF-β1-stimulated MRC-5 cells. MRC-5 cells were transfected with scramble or MYH9 siRNA (50 nM); 24 h after transfection, the cells were starved overnight. MRC-5 cells were treated with/without 2.5 ng/ml of TGF-β1 for 1 h. MRC-5 cell protein samples were prepared and assayed by Western blot analysis for determing the levels of Smad2/3 phosphorylation (n = 3, control group vs. TGF-β1 group: *p < 0.05; Scramble siRNA group vs. MYH9 siRNA group: NS).