FIGURE 3.

Lercanidipine inhibited the phosphorylation of STAT1. (A) NCI-H1299 cells were analyzed by Western blot for P-STAT1, STAT1 and PD-L1 proteins after the treatment of indicated concentrations of Lercanidipine (2.5, 5, and 10 μM) for 24 h. (B) The P-STAT1, STAT1 and PD-L1 proteins in NCI-H1299 cells were evaluated with or without Lercanidipine in the presence of IFNγ (10 ng/ml) for 0, 3, 6, 9, 12, and 24 h. LE, long exposure; SE, short exposure. (C) The expression of PD-L1 was measured in NCI-H1299 cells transfected with siSTAT1 treated with or without IFNγ (10 ng/ml) and Lercanidipine (10 μM).