Fig. 1.

Romidepsin inhibited proliferation, induced cell cycle arrest and increased apoptosis in CT26 and MC38 cells. a The effects of romidepsin on the cell cycle were evaluated in murine colon cancer cell lines. CT26 and MC38 cells were treated with or without romidepsin (10 μM for CT26 cells and 40 nM for MC38 cells) for 24 h and then cultured with Brdu (10 ul/mL) for 4 h. The cell cycle profile was measured by flow cytometry to detect the incorporation of Brdu into DNA during cell proliferation. The bar chart displays the percentages of cells in each phase. Three replicates (n = 3) were set for each experimental group. b Expression levels of PCNA were determined by western blot with anti-PCNA antibodies. The histogram was used to analyze the fold change of sample grayscale from western blot result. c Apoptosis was examined using an Annexin V/PI assay. The cells in the region of Q2 and Q4 were defined as apoptotic cells. Three replicates (n = 3) were set for each experimental group. d The level of caspase-3 was determined by Western blot. The histogram was used to analyze the fold change of sample grayscale from western blot result (*P < 0.05, **P < 0.01)