Fig. 3.

Impact of leukotriene C4/D4 on sMICA production by shedding mMICA via ADAM9 in HCC cells. Cell viabilities (a) and sMICA levels (b) were determined by CCK8 assay and ELISA, respectively, after treating HepG2 and PLC/PRF/5 with leukotriene C4/D4. (c) HepG2 and PLC/PRF/5 cells were treated with 100 µM leukotriene C4/D4 for 96 h, and mMICA level was assessed by flow cytometry. The isotype controls are shown as gray histograms (no treatment, leukotriene C4, and leukotriene D4 shown in black, blue, and red, respectively), and fluorescence intensity and cell counts are indicated on the X and Y axis, respectively. d The effect of leukotriene D4 for 48 h on mMICA level in HepG2 cells was examined in the presence of siCtrl (0 and 100 µM in black and blue, respectively) or siADAM9 (100 µM shown in red) by flow cytometry. The isotype controls are shown as gray histograms and fluorescence intensity and cell counts are indicated on the X and Y axis, respectively. *P < 0.05; **P < 0.01; ***P < 0.005. Error bars represent SEM. Representative data from three independent experiments with consistently similar results are shown