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. 2021 Jan 26;11:2231. doi: 10.1038/s41598-021-81819-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Low-dose recombinant AMH (LR-AMH) promotes cell viability in COV434-AMHRII, SKOV3-AMHRII, OVCAR8 and KGN cells. (a) Cell viability (MTS assay) and (b) AKT phosphorylation (western blotting) after incubation with 0.8 to 25 nM LR-AMH for 3 days. (c) AMH expression at 48 h after transfection of siRNAs against AMH, and (d) Effect of siRNA-mediated AMH silencing on cell viability at day 3 post-transfection. Figure shows a representative experiment out of 2 to 4 experiments performed depending on the cell line (mean ± SEM of 3 replicate wells) *p < 0.05; ***p < 0.001. For western blot figure preparation, all proteins were analyzed on the same gel and membrane. After blotting, the lines corresponding to the different proteins were cut and may have been exposed for different lengths of time because the objective was not to compare the different proteins with each other, but to see the effect of AMH (b) or of siRNA (c) on the expression of each protein.