Table 2.
Formulations and properties of ALL-loaded CS/STPP NPs prepared according to 24 full factorial design.
| Formula code | Coded levels of | Q (mg) | DEE % | PDI | Particle size (nm) | ZP (mV) | |||
|---|---|---|---|---|---|---|---|---|---|
| X1 | X2 | X3 | X4 | ||||||
| F-1 | − | + | + | − | 4.91 ± 0.04 | 49.08 ± 0.39 | 0.178 ± 0.04 | 610.97 ± 17.01 | 22.97 ± 0.98 |
| F-2 | − | + | − | − | 3.51 ± 0.05 | 35.14 ± 0.54 | 0.264 ± 0.01 | 665.27 ± 18.85 | 24.83 ± 0.60 |
| F-3 | + | + | + | − | 2.91 ± 0.25 | 29.11 ± 2.53 | 0.116 ± 0.04 | 676.13 ± 15.21 | 34.53 ± 2.31 |
| F-4 | + | + | − | − | 3.16 ± 0.04 | 31.58 ± 0.41 | 0.171 ± 0.04 | 747.53 ± 33.22 | 29.90 ± 0.62 |
| F-5 | + | − | + | − | 5.35 ± 0.18 | 53.51 ± 1.77 | 0.212 ± 0.05 | 681.03 ± 20.57 | 29.63 ± 1.06 |
| F-6 | + | − | − | − | 4.51 ± 0.26 | 45.12 ± 2.58 | 0.282 ± 0.04 | 735.70 ± 2.25 | 29.33 ± 1.15 |
| F-7 | − | − | + | − | 3.60 ± 0.09 | 36.02 ± 0.93 | 0.230 ± 0.04 | 465.07 ± 20.51 | 28.40 ± 1.44 |
| F-8 | − | − | − | − | 4.69 ± 0.18 | 46.85 ± 1.85 | 0.251 ± 0.06 | 507.90 ± 13.25 | 28.33 ± 0.21 |
| F-9 | + | − | + | + | 6.26 ± 0.03 | 48.12 ± 0.21 | 0.290 ± 0.02 | 508.30 ± 13.35 | 35.70 ± 0.82 |
| F-10 | + | − | − | + | 5.12 ± 0.07 | 39.40 ± 0.58 | 0.346 ± 0.01 | 512.73 ± 40.75 | 29.47 ± 0.64 |
| F-11 | − | − | + | + | 3.03 ± 0.29 | 23.34 ± 2.27 | 0.320 ± 0.06 | 479.07 ± 7.53 | 28.57 ± 0.42 |
| F-12 | − | − | − | + | 4.20 ± 0.13 | 32.34 ± 1.01 | 0.389 ± 0.08 | 493.50 ± 15.81 | 27.47 ± 0.57 |
| F-13 | − | + | − | + | 1.87 ± 0.07 | 14.42 ± 0.55 | 0.478 ± 0.04 | 604.37 ± 13.24 | 28.33 ± 0.91 |
| F-14 | + | + | − | + | 4.30 ± 0.42 | 32.93 ± 2.99 | 0.281 ± 0.05 | 791.67 ± 27.37 | 34.27 ± 0.93 |
| F-15 | − | + | + | + | 3.81 ± 0.24 | 29.33 ± 1.86 | 0.413 ± 0.09 | 478.13 ± 15.76 | 28.27 ± 0.75 |
| F-16 | + | + | + | + | 5.22 ± 0.08 | 40.18 ± 0.61 | 0.235 ± 0.03 | 711.43 ± 16.83 | 35.80 ± 0.92 |
Each value represents the mean ± SD (n = 3). X1, X2, X3, X4 are CS concentration, STPP concentration, CS:STPP volume ratio and ALL amount, respectively.