Table 4.

Particle size, PDI, ZP and drug retention % of ALL-loaded CS/STPP NPs aqueous dispersions (F-9) stored at refrigeration (4 ± 1 °C) and ambient conditions (see Table 2 for F-9).

Storage time	Evaluation parameters
	Refrigeration conditions (4 ± 1 °C)				Ambient conditions
	Particle size (nm)	PDI	ZP (mV)	Drug retention (%)	Particle size (nm)	PDI	ZP (mV)	Drug retention (%)
Zero time	508.30 ± 13.35	0.290 ± 0.02	+ 35.70 ± 0.82	100.00 ± 0.00	508.30 ± 13.35	0.290 ± 0.02	35.70 ± 0.82	100.00 ± 0.00
1 month	499.17 ± 0.93	0.282 ± 0.03	+ 33.60 ± 1.87	98.95 ± 5.29	501.57 ± 64.48	0.307 ± 0.02	+ 31.47 ± 0.99*	96.24 ± 3.62
3 months	504.60 ± 4.66	0.301 ± 0.02	+ 33.07 ± 1.11	98.202 ± 2.78	589.70 ± 8.97*#	0.509 ± 0.01*#	+ 14.80 ± 0.95*#	92.88 ± 3.33

Each value represents the mean ± SD (n = 3).

*Significant at p < 0.05 monthly vs. initial.

^#Significant at p < 0.05 refrigeration vs. ambient conditions after 3 months.