Skip to main content
. 2021 Jan 26;4:119. doi: 10.1038/s42003-020-01642-5

Fig. 2. Paracrine impact of secreted miR-424.

Fig. 2

A Experimental design for phenotypic analysis. B miR-424 levels in RWPE-1 and LNCaP donor cells stably expressing miR-424 or control plasmid. C miR-424 levels in recipient cells (RWPE-1) and (LNCaP) following the addition of conditioned medium (CM) from donor cells with (CM-424) or without (CM-CTRL) stable expression of miR-424. D miR-424 level in RWPE-1 cells transfected with pre-miR-424 (424) or negative control pre-miRNA (CTRL). E miR-424 transfer in recipient RWPE-1 cells following incubation with CM from control or pre-miR-424 transfected RWPE-1 cells. F Tumor-sphere forming efficiency (SFE) of RWPE-1 recipient cells incubated with CM from RWPE-1 and LNCaP donor cells with (CM-424) or without (CM-CTRL) miR-424 expression. G and H Colony (G) and tumor-sphere formation (H) after supplementation of CM from control or pre-miR-424 transfected RWPE-1 cells. Representative images are shown at bottom of panels F and G. I Cell migration capacity measured in wound-healing assay of RWPE-1 cells after supplementation of CM from control and pre-miR-424 transfected RWPE-1 cells. Right: quantification of wound width closure percentage. *p ≤ 0.05, **p ≤ 0.01 by two-tailed Student’s t-test.