Table 1.
Detailed results of statistical analysis per figure.
| Figure | Test | Post-hoc comparisons |
|---|---|---|
| 1B | Fisher exact test | n/a |
| Naïve vs BDNF: P < 0.0001 | ||
| 1C | Brown-Forsythe | Games-Howell's multiple comparison test |
| F(2, 4598) = 3373, P < 0.0001 | Silent vs naïve: P < 0.0001; silent vs BDNF: P < 0.0001; naïve vs BDNF: P < 0.0001 | |
| Welch's ANOVA | ||
| W(2, 2892) = 5458, P < 0.0001 | ||
| 1D | Brown-Forsythe | Games-Howell's multiple comparison test |
| F(2, 4280) = 1737, P < 0.0001 | Silent vs naïve: P < 0.0001; silent vs BDNF: P < 0.0001; naïve vs BDNF: P < 0.0001 | |
| Welch's ANOVA | ||
| W(2, 2502) = 2032, P < 0.0001 | ||
| 1F | Kruskal–Wallis test | Dunn's multiple comparison test |
| KW = 54.23, P < 0.0001 | Silent vs naïve: P < 0.0001; silent vs BDNF: P < 0.0001; naïve vs BDNF: P = 0.0939 | |
| 1G | Kruskal–Wallis test | Dunn's multiple comparison test |
| KW = 54.35, P < 0.0001 | Silent vs naïve: P < 0.0001; silent vs BDNF: P < 0.0001; naïve vs BDNF: P = 0.0732 | |
| 1H | Kruskal–Wallis test | n/a |
| KW = 4.507, P = 0.1051 | ||
| 1I | Kruskal–Wallis test | Dunn's multiple comparison test |
| KW = 15.04, P = 0.0005 | Silent vs naïve: P = 0.0087; silent vs BDNF: P = 0.0003; naïve vs BDNF: P = 0.4002 | |
| 1J | Kruskal–Wallis test | n/a |
| KW = 2.810, P = 0.2454 | ||
| 2B | Two-way RM ANOVA | Sidak's multiple comparison test |
| Interaction: F(1, 77) = 29.27, P < 0.0001, 10.65% total variation | Control vs octanol (Decrease): P = 0.4469; Control vs octanol (Increase): P < 0.0001 | |
| Group effect: F(1, 77) = 0.0392, P = 0.8437, 0.028% total variation | ||
| Treatment effect: F(1, 77) = 14.31, P = 0.0003, 5.208% total variation | ||
| Subjects (matching) effect: F(77, 77) = 1.994, P = 0.0014, 55.89% total variation | ||
| 2C | Two-way RM ANOVA | Sidak's multiple comparison test |
| Interaction: F(1, 77) = 13.95, P = 0.0004, 7.126% total variation | Control vs octanol (Decrease): P = 0.9638; Control vs octanol (Increase): P < 0.0001 | |
| Group effect: F(1, 77) = 7.287, P = 0.0085, 4.105% total variation | ||
| Treatment effect: F(1, 77) = 11.50, P = 0.0011, 5.875% total variation | ||
| Subjects (matching) effect: F(77, 77) = 1.102, P = 0.3349, 43.38% total variation | ||
| 2D | Two-way RM ANOVA | Sidak's multiple comparison test |
| Interaction: F(1, 77) = 13.63, P = 0.0004, 7.764% total variation | Control vs octanol (Decrease): P = 0.1876; Control vs octanol (Increase): P = 0.0013 | |
| Group effect: F(1, 77) = 0.080, P = 0.7780, 0.049% total variation | ||
| Treatment effect: F(1, 77) = 1.725, P = 0.1929, 0.983% total variation | ||
| Subjects (matching) effect: F(77, 77) = 1.078, P = 0.3709, 47.28% total variation | ||
| 3D | Mann–Whitney test | n/a |
| U = 606.5, P = 0.0010 | ||
| 3E1 | Wilcoxon matched-pairs signed rank test | n/a |
| W = -1373, P < 0.0001 | ||
| 3E2 | Two-way RM ANOVA | Sidak's multiple comparison test |
| Interaction: F(1, 58) = 37.70, P < 0.0001, 6.086% total variation | Control vs octanol (Decrease): P < 0.0001; Control vs octanol (Increase): P = 0.0450 | |
| Group effect: F(1, 58) = 38.23, P < 0.0001, 29.22% total variation | ||
| Treatment effect: F(1, 58) = 83.93, P < 0.0001, 13.55% total variation | ||
| Subjects (matching) effect: F(58, 58) = 4.736, P < 0.0001, 44.34% total variation | ||
| 4A-B | Paired t tests | n/a |
| Control vs 0 Ca2+: Amplitude P > 0.05; Frequency P > 0.05 | ||
| Control vs Cd2+: Amplitude P > 0.05; Frequency P > 0.05 | ||
| Control vs Ni2+: Amplitude P > 0.05; Frequency P < 0.001 | ||
| Control vs nitrendipine: Amplitude P > 0.05; Frequency P < 0.001 | ||
| Control vs ω-conotoxin: Amplitude P > 0.05; Frequency P < 0.001 | ||
| Control vs TTX: Amplitude P > 0.05; Frequency P > 0.05 | ||
| Control vs riluzole: Amplitude P > 0.05; Frequency P < 0.001 | ||
| Control vs NBQX: Amplitude P > 0.05; Frequency P > 0.05 | ||
| Control vs D-AP5: Amplitude P < 0.001; Frequency P > 0.05 | ||
| Control vs kynurenic acid: Amplitude P > 0.05; Frequency P > 0.05 | ||
| Control vs GABA: Amplitude P > 0.05; Frequency P > 0.05 | ||
| 5C2 | Brown-Forsythe | Games-Howell's multiple comparison test |
| F(3, 2916) = 311.1, P < 0.0001 | Control vs BDNF (Delay): P = 0.4264; Control vs BDNF (Tonic): P < 0.0001 | |
| Welch's ANOVA | ||
| W(3, 2862) = 504.2, P < 0.0001 | ||
| 5D2 | Brown-Forsythe | Games-Howell's multiple comparison test |
| F(3, 1761) = 419.3, P < 0.0001 | Control vs BDNF (Delay): P < 0.0001; Control vs BDNF (Tonic): P < 0.0001 | |
| Welch's ANOVA | ||
| W(3, 2791) = 541.8, P < 0.0001 | ||
| 500 | Brown-Forsythe | Games-Howell's multiple comparison test |
| F(3, 2550) = 159.1, P < 0.0001 | Control vs BDNF (Delay): P < 0.0001; Control vs BDNF (Tonic): P = 0.0012 | |
| Welch's ANOVA | ||
| W(3, 2759) = 162.3, P < 0.0001 | ||
| 5F2 | Brown-Forsythe | Games-Howell's multiple comparison test |
| F(3, 2027) = 14.5, P < 0.0001 | Control vs BDNF (Delay): P = 0.1284; Control vs BDNF (Tonic): P < 0.0001 | |
| Welch's ANOVA | ||
| W(3, 2860) = 27.93, P < 0.0001 | ||
| S1B | Kruskal–Wallis test | Dunn’s multiple comparison test |
| KW = 44.87, P < 0.0001 | Silent vs naïve: P < 0.0001; silent vs BDNF: P = 0.0002; naïve vs BDNF: P = 0.0020 | |
| S4A2 | Brown-Forsythe | Games-Howell's multiple comparison test |
| F(3, 71793) = 1122, P < 0.0001 | Control vs BDNF (Delay): P < 0.0001; Control vs BDNF (Tonic): P < 0.0001 | |
| Welch's ANOVA | ||
| W(3, 54420) = 2011, P < 0.0001 | ||
| S4A4 | Brown-Forsythe | Games-Howell's multiple comparison test |
| F(3, 29988) = 851.4, P < 0.0001 | Control vs BDNF (Delay): P < 0.0001; Control vs BDNF (Tonic): P < 0.0001 | |
| Welch's ANOVA | ||
| W(3, 18137) = 2294, P < 0.0001 | ||
| S4B2 | Brown-Forsythe | Games-Howell's multiple comparison test |
| F(3, 55836) = 870.5, P < 0.0001 | Control vs BDNF (Delay): P < 0.0001; Control vs BDNF (Tonic): P < 0.0001 | |
| Welch's ANOVA | ||
| W(3, 54028) = 850.3, P < 0.0001 | ||
| S4B4 | Brown-Forsythe | Games-Howell's multiple comparison test |
| F(3, 22611) = 438.9, P < 0.0001 | Control vs BDNF (Delay): P < 0.0001; Control vs BDNF (Tonic): P < 0.0001 | |
| Welch's ANOVA | ||
| W(3, 17636) = 1298, P < 0.0001 | ||
| S4C2 | Brown-Forsythe | Games-Howell's multiple comparison test |
| F(3, 72912) = 203.0, P < 0.0001 | Control vs BDNF (Delay): P < 0.0001; Control vs BDNF (Tonic): P < 0.0001 | |
| Welch's ANOVA | ||
| W(3, 51340) = 169.4, P < 0.0001 | ||
| S4C4 | Brown-Forsythe | Games-Howell's multiple comparison test |
| F(3, 26038) = 26.33, P < 0.0001 | Control vs BDNF (Delay): P = 0.0062; Control vs BDNF (Tonic): P = 0.6416 | |
| Welch's ANOVA | ||
| W(3, 14707) = 27.89, P < 0.0001 | ||
| S4D2 | Brown-Forsythe | Games-Howell's multiple comparison test |
| F(3, 76383) = 171.1, P < 0.0001 | Control vs BDNF (Delay): P < 0.0001; Control vs BDNF (Tonic): P < 0.0001 | |
| Welch's ANOVA | ||
| W(3, 51987) = 167.3, P < 0.0001 | ||
| S4D4 | Brown-Forsythe | Games-Howell's multiple comparison test |
| F(3, 26111) = 62.09, P < 0.0001 | Control vs BDNF (Delay): P < 0.0001; Control vs BDNF (Tonic): P < 0.0001 | |
| Welch's ANOVA | ||
| W(3, 16008) = 62.78, P < 0.0001 |