Figure 2.

(A) Set-up of RNA-seq experiment. hiPSCs transduced with two lentiviral vectors expressing rtTA and Ngn2 are treated with doxycycline to initiate differentiation into iNeurons up to DIV38. On DIV2, rat astrocytes are added to the culture to support neuron differentiation. The experiments were performed both on the microfluidic chip and on a 24-well plate. On DIV38, RNA was isolated from two replicates per culture condition. Separately, HUVECs were cultured on the microfluidic chip and on a 24-well plate as well. After 24 h, RNA was isolated from two replicates per culture condition. (B–D) Heatmaps of Euclidean distances between samples based on gene expression profiles, generated per cell type (HUVEC, iNeurons, and astrocytes). Within each cell type, samples cluster according to culture condition.