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. 2021 Jan 26;11:2187. doi: 10.1038/s41598-021-81276-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression level analyses. (a) qRT-PCR was used to measure the relative gene expression of the different AtG3BPs in roots, stem, flower, silique, and rosette leaves. Transcript levels were calculated using the 2^-∆Ct method with AtActin-2 as the endogenous control. The error bar represents the standard error. (b) Expression ratio of AtG3BP genes in phloem tissue compared to non-phloem tissue in A. thaliana leaves extracted by laser dissection of AtSUC2promoter::GFP plants. Gene expression was normalized to AtActin-2 and expression levels were calculated using the 2^−∆Ct method.