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. 2021 Jan 26;11:2187. doi: 10.1038/s41598-021-81276-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression level analyses. (a) Transcript abundance in TuMV-infected leaf tissue was measured and compared to non-infected tissue to determine the regulation of the expression level of the different AtG3BPs, AtActin-2 served as the internal control. The asterisks indicate significance measured by two-tailed t-test (** = p < 0.01; *** = p < 0.005) (b) qRT-PCR analysis for verification of RNAseq data. AtG3BP-4 expression level in TuMV infection sites 2 dpi was measured and compared to mock-inoculated tissue.