Fig. 1. ZIKV infects placental trophoblasts.
a Immunofluorescence staining of Viral E protein in HTR-8 (HTR-8/SVneo) cells 72 h postinfection with 0.1 multiplicity of infection (MOI) recombinant MR strain (r-MRV) or PRVABC-59 (PRV) or uninfected vehicle cells. Nuclei were stained with DAPI and ZIKV E protein stained with rabbit polyclonal primary antibody and Alexaflour 488-conjugated ant-rabbit secondary antibody. b Western blot analysis showing increased expression of viral E protein, 16–24 h postinfection when compared to uninfected vehicle cells in JEG-3, JAR, and HTR-8. β-Actin was used as a loading control.