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. 2021 Jan 26;7:24. doi: 10.1038/s41420-020-00379-8

Table 1.

Primer details.

Primer Forward primer Reverse primer Product length
XBP1 5′AAACAGAGTAGCAGC TCAGACTGC 3′ 5′TCCTTCTGGGTAGAC CTCTGGGAG 3′ Unspliced forms’ ∼474 bp cleaved by the restriction endonuclease (Pst1) two products are around 296 bp and 183 bp; spliced forms lack restriction enzyme site ∼448 bp
GAPDH 5′AATCCCATCACCATC TTCCA 3′ 5′TTCACACCCATGACG AAC AT 3′ ∼194 bp
18srRNA 5′CGTTCTTAGTTGGTG GAGCG 3′ 5′CGCTGAGCCAGT CAG TGTAG 3′ ∼121 bp