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. 2021 Jan 26;12(1):128. doi: 10.1038/s41419-021-03399-z

Fig. 7. BRD4 serves as a functional target of miR-211-5p.

Fig. 7

A Bioinformatic analysis indicated the potential target sites for miR-211-5p in BRD4 and SNHG18. B Luciferase reporter assay showed that miR-211-5p repressed the expression of the BRD4 3’-UTR reporter. Overexpression of SNHG18 rescued miR-211-5p-mediated repression of BRD4 3’-UTR reporter. n = 3. C Quantitative PCR analysis of BRD4 mRNA in A549 and H1299 cells transfected with control mimic or miR-211-5p mimic. *P < 0.05 vs. control mimic, Student’s t-test. n = 3. D Western blot analysis of BRD4 protein expression in NSCLC cells transfected with empty vector or BRD4-expressing vector. E, F Assessment of E cell proliferation and F invasion in NSCLC cells transfected with indicated constructs. *P < 0.05. n = 3. In all panels in this figure, one-way ANOVA and Bonferroni’s tests were performed unless otherwise described.