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. 2021 Jan 13;11:610568. doi: 10.3389/fmicb.2020.610568

FIGURE 4.

FIGURE 4

EV-A71 enterovirus VP1-280T bound to a human neuroblastoma cell line SH-SY5Y better than the other virus VP1-280A. (A) The binding assay was performed as described in section “Materials and Methods.” SH-SY5Y and HTB10: human neuroblastoma cell lines, RD: a human rhabdomyosarcoma cell line. *Student’s t-test statistical analysis p < 0.05. (B) Comparisons of the SCARB2 expression levels among different cells infected with viruses VP1-280T vs. VP1-280A. Western blot analysis was performed for the detection of SCARB2 proteins using an anti-SCARB2 antibody. IMR32: human neuroblastoma cell line, 3T3 and L929 are mouse fibroblast cell lines. Mock, uninfected culture; GAPDH, an internal control.