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. 2021 Jan 13;14:598198. doi: 10.3389/fncel.2020.598198

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Copyright © 2021 Tang, Qin, Li, Chen, Ulloa, Zhu, Liu, Gong, Zhao, Wang, Li, Guo, Xu and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HTWP acupuncture activates P2RX7 neurons in the ventral periaqueductal gray. (A–C) Confocal and co-localization analyses of P2RX7, P2RX3, and TRPV1 with c-fos and DAPI. (D–F) HistoFAXS tissue analysis of c-fos staining mean intensity vs. area (μm²), and quantitative analysis by image scanning of co-localization of c-fos with P2RX7, P2RX3, and TRPV1 in the left vPAG of sham and TBI rats treated with or without HTWP acupuncture (hand Acu) (n = 3–4/group; one-way ANOVA). Error bars represent mean + SEM; ^▴P < 0.05 vs. TBI.