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. 2021 Jan 13;11:545654. doi: 10.3389/fmicb.2020.545654

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Copyright © 2021 Ceresa, Rinaldi, Tessarolo, Maniglio, Fedeli, Tambone, Caciagli, Banat, Diaz De Rienzo and Fracchia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Scanning electron microscopy images of the dual-species biofilms formed on the silicone discs surface at 72 h. C. albicans–S. aureus on the left column and C. albicans–S. epidermidis on the right column. Different surface pre-coating treatments are presented: untreated controls (top row), rhamnolipid R89BS (middle raw), and sophorolipid SL18 (bottom row) treated discs. Insets present a lower magnification of the corresponding image to appreciate both macrostructural arrangement of the biofilm on the surface and micro-structural architecture of the two species in each biofilm sample. Original magnification: 4000x (1000x for the insets).