Figure 2.
Treatment of human chondrosarcoma HTB-94 cells with berberine inhibited cell proliferation. HTB-94 cells were treated with the indicated concentrations of berberine (0–100 mM) for 24 h or 100 µM berberine for various time periods (6, 12, or 24 h). (A and C) Photographs of HTB-94 cells were taken with a phase-contrast microscope (magnification, A: 200×, B: 100×). (B and D) Cell proliferation was estimated using the methyl thiazole tetrazolium (MTT) assay. The photomicrograph data are presented as the results of a typical experiment, and the graphical data are expressed as mean values ± standard deviation. *p < 0.05 compared to the control.