Figure 5.

Berberine induced p53/p21-dependent G₂/M phase arrest in HTB-94 cells. HTB-94 cells were untreated (0) or treated with 100 µM of berberine for the indicated times (10 or 30 min or 1, 3, 6, 12, or 24 h). (A) Expression of p53, p21, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected using Western blot analysis. GAPDH was used as loading control (upper panel). The relative amounts of p53 and p21 were quantified by a densitometric analysis (ImageJ, lower panel). HTB-94 cells were treated with the indicated various concentrations of berberine (0, 10, 30, 50, or 100 mM) for 24 h. (B) Expression of p53, p21, and GAPDH was detected using Western blot analysis. GAPDH was used as loading control (upper panel). The relative amounts of p53 and p21 were quantified by a densitometric analysis (ImageJ, lower panel). Western blot data are presented as results of a typical experiment, and graphical data are expressed as the mean ± standard deviation. *p < 0.05 compared to the control.