Fig. 2. Cytotoxic effects induced by RSV and DTX in LNCaP cells. Cells were treated with indicated concentrations of RSV and DTX, otherwise RSV (20 μM) and DTX (2 nM) alone or in combination for 48 h. (A) Cell cycle distribution was determined by flow cytometry following PI (20 μg/mL) staining. (B) Apoptotic cell fraction was analyzed using Annexin V-PE binding assay. (C) The levels of DNA damage response proteins were assessed by Western blotting.

RSV, resveratrol; DTX, docetaxel; PI, propidium iodide; ATM, ataxia telangiectasia mutated; ATR, ataxia telangiectasia mutated and RAD3-related.